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New Media Medicine Forums - Medical News

New Media Medicine Forums - Medical News

VivienLauYW, Maria Wong - Mutation and Expression Analysis of PTEN in Non-small Cell

Posted: 11 Dec 2014 05:51 AM PST

VivienLauYW – The University of Hong Kong Medical Sciences Pathology Pharmacology MMedSc
Mutation and Expression Analysis of PTEN in Non-small Cell Lung Cancer From Non-smokers
Abstract
This MMedSc dissertation studied the genetic variations and epigenetic variations of PTEN (phosphatase and tensin homolog deleted on chromosome 10) in relation to Adenocarcinoma (AD) in particular the protein expression level of PTEN in non-smoking non-small cell lung cancer (NSCLC) patient.
PTEN gene is a tumor suppressor gene (TSG) residing at the locus10q23.3. A decrease in PTEN protein level induces the accumulation of lipid phosphoinosital 3,4,5-triphosphate (PIP3) and cell proliferation. Samples of 121 NSCLC patients were subjected to immunohistocheistry staining ro determine the association between PTEN protein expression level and clinicopathological feature of NSCLC. Out of the 121 NSCLC samples, 32 samples with no mutation in other known oncogenes such as EGFR and KRAS were screened for PTEN protein expression level, PTEN promoter hypermethylation and PTEN gene mutation. Direct sequencing on PTEN gene and methylation-specific PCR (MSP) were used as a detection method in this study. Wild type EGFR expression was related or a loss or reduced PTEN expression in NSCLC patient (P=0.006), and PTEN promoter non-methylation was associated with AD in NSCLC patients. Supervisor: Doctor Maria Wong

VivienLauYW, Carl Douglas, Clarice Souza - Peroxisomal Enzymes Acyl-CoA ligase-like

Posted: 11 Dec 2014 05:48 AM PST

VivienLauYW – The University of British Columbia UBC Honor of Developmental Biology Directed Studies The Final Version of Vivien Lau YW's Written Research Report. Sub-cellular Localization of Putative Peroxisomal Enzymes Detected By CFP-hybrid-gene Construct Transiently Expressed in Arabidopsis thaliana Carrying YFP-tagged Peroxisome. We accept this thesis as conforming to the required standard. A plant model was used to demonstrate the predictability of function of protein by its protein folding deminstion imprinted by its amino acid sequence. Acyl-CoA ligase-like (ACLL) genes were studied in my project. The ACLL gene family was discovered by comparing the amino acid sequence of a well-studied gene, 4-coumarat: CA ligase (4CL) of Arabidopsis thaliana, with all the amino acid sequences of Arabidopsis thaliana (Arabidopsis) and Populus trichocarpa (Poplar) in Genebank. To study ACLL proteins, we located ACLL proteins in intact plant calls. One noticeable difference between 4CL and ACLL proteins is 4CL proteins have no peroxisomal targeting signal (PTS1) an ACLL proteins have PTS1 at their C-terminus. ACLL proteins are likely localizing in the peroxisome. However, to prove this theory the true localizations of ACLL proteins have to be determined by experiment. In this project, I cloned the ACLL genes of arabidopsis and poplar and merge to a Cyan Fluorescent Protein (CFP)-hybrid gene construct. Peroxisomal marker is a Yellow Fluorescent Protein (YFP)-tagged peroxisome. ACLL proteins are strongly suggested to be the transportation signal to peroxisome. This study proved the function of similar amino acid sequence across specious. Supervisor: Professor Carl Douglas and Clarice Souza

VivienLauYW, Singh S - External Cadmium on Barley Plant (Hordeum vulgare)

Posted: 11 Dec 2014 05:44 AM PST

VivienLauYW – The University of British Columbia UBC Honor of Developmental Biology Directed Studies The Final Version of Vivien Lau YW's Written Research Report. The Effect o different Concentration of External Cadmium on Barley Plant (Hordeum vulgare) Pretreated by Iron-deficient Nutrient and Iron-sufficient Nutrient (+/- Fe), Salicylic Acid (SA), Abscisic Acid (ABA), Benzyl-amino purine (BAP), and Jasmonic Acid (JA) is studied by the Height of plant, the Chlorophyll Level, the Total Protein Level, the Peroxidase Activity, the SDS-PAGE and the Internal Cadmium of the Pretreated Barley Plant. This experiment found that the –Fe pretreated barley plant can be used in the phytoremediation of Cadmium contaminated land while SA, JA and high concentration of ABA can be used to allow barley to grow on Cadmium contaminated land and the internal Cadmium of those barley product may be edible without contaminating food cycle. Supervisor: Dr. Singh S

VivienLauYW - Medical Sciences University of Hong Kong MMedSc HKU

Posted: 11 Dec 2014 05:39 AM PST

VivienLauYW – Medical Sciences University of Hong Kong MMedSc HKU
Flow Cytometry in the detection of Apoptosis
Extract DNA in pH7.8
Fluorescence store at 4 Degree Celsius in dark.
Apoptotic process is around 6 hour.

VivienLauYW - Focus Group Research on User perspective of Fecal Immunochemical Test

Posted: 11 Dec 2014 05:37 AM PST

VivienLauYW – Public Health The Chinese University of Hong Kong
Jockey Club School of Public Health and Primary Care Department of Health
Focus Group Research on User perspective of Fecal Immunochemical Test (FIT) for Colorectal Cancer Screening.

VivienLauYW –Jockey Club School of Public Health and Primary Care Public Health The Chinese University of Hong Kong Department of Health
Focus Group Research on User perspective of Fecal Immunochemical Test (FIT) for Colorectal Cancer Screening.

VivienLauYW –Department of Health Public Health The Chinese University of Hong Kong
Jockey Club School of Public Health and Primary Care
Focus Group Research on User perspective of Fecal Immunochemical Test (FIT) for Colorectal Cancer Screening.

The Surveillance and Epidemiology Brand (SEB) of the Cancer for Health Protection, Department of Health (DH) has commissioned the CUHK Jockey Club Bowel Cancer Education Centre to conduct a focus group study. Total 86 respondents were interviewed. This study provided information to enable the Department of Health to incorporate Fecal immunochemical Test in user-friendliness of public cancer screening.

VivienLauYW - A Cross-sectional Study of Insomnia and Daily Nutrient Intake.

Posted: 11 Dec 2014 05:35 AM PST

VivienLauYW – The Chinese University of Hong Kong
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy Public Health and Psychiatry
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy Public Health
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy Psychiatry
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy Nutrition
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

VivienLauYW – The Chinese University of Hong Kong
Master of Philosophy Dietician
A Cross-sectional Study of Hong Kong Chinese Population Investigating the Association of Insomnia and Daily Nutrient Intake.

Abstract
The association of nutrients and sleep is a debatable question in sleep sciences. This study attempts to address the association between nutrients and insomnia. This literature is part of my dissertation. Chapter one addressed the history of sleep and insomnia; Chapter two to four addressed the age, mood, pain, and sleep hygiene, caffeine, drug an alcohol effect on sleep. Nutrients effect on insomnia was discussed in Chapter five and six. Insomnia is a common sleep disorder and a major public health issue. Insomnia could be classified into three subtypes: Difficulty in Initiating Sleep (DIS), Difficulty in Maintaining Sleep (DMS), and Early Morning Wakening (EMW). My study was conducted in 138 adolescents and 173 adults in a community-based cross-sectional observation. Information of daily nutrient intake was obtained by a self-administrated 3-day food dietary, the assessment of insomnia symptom was obtained by a standard sleep questionnaire such as Insomnia Severity Index (ISI), and the assessment of anxiety and depression was obtained by commonly used scale such as Hospital Anxiety and Depression Scale (HADS). Associations are found between insomnia and nutrient components. Decreased intake of saturated fat, carbohydrate, vitamin A, vitamin D, and vitamin E are associated with insomnia subtype DIS and overall insomnia syndrome in adult and in adolescent. Decreased intake of saturated fat is found associated with insomnia subtype DMS and decreased intake of carbohydrate is found associated with insomnia subtype EMW.

VivienLauYW - The Sick role of Juvenile Rheumatoid Arthritis(JRA) Patients and Parent

Posted: 11 Dec 2014 05:31 AM PST

VivienLauYW – Master of Public Health The Chinese University of Hong Kong
The Sick role of Juvenile Rheumatoid Arthritis (JRA) Patients and their Parents.

VivienLauYW – Master of Public Health The Chinese University of Hong Kong
Medical Sociology
The Sick role of Juvenile Rheumatoid Arthritis (JRA) Patients and their Parents.

VivienLauYW - The Attitude of New Licensed and Old Licensed Drivers Towards the New T

Posted: 11 Dec 2014 05:29 AM PST

VivienLauYW – Master of Public Health The Chinese University of Hong Kong
How does the new traffic regulation affect new/ old licensed drivers?

VivienLauYW – Master of Public Health The Chinese University of Hong Kong
The Attitude of New Licensed and Old Licensed Drivers Towards the New Traffic Regulation of Drink Driving of Hong Kong in 2009.

VivienLauYW – Master of Public Health The Chinese University of Hong Kong Quantitative Research
The Attitude of New Licensed and Old Licensed Drivers Towards the New Traffic Regulation of Drink Driving of Hong Kong in 2009.

Abstract
New traffic regulation was passed in Hong Kong in 2009 on an anti-drunk driving/ Knowing what and how the new traffic regulation impacts on the new and existing licensed drivers, as well as under what circumstances do drivers forget anti-drunk driving will be important in the commence of anti-drunk driving regulation of Hong Kong. This study invited 176,000 licensed drivers in Hong Kong to participate in focus groups on drink driving.

VivienLauYW - Health Risk Factors of University Students in Hong Kong and Shantou

Posted: 11 Dec 2014 05:27 AM PST

VivienLauYW – Master of Public Health The Chinese University of Hong Kong
A Cross-sectional Study of Associations between Adverse Drinking Behaviors and Health Risk Factors of University Students in Hong Kong and Shantou (Final Report).

VivienLauYW – Master of Public Health The Chinese University of Hong Kong Health Risk Factors of University Students in Hong Kong and Shantou (Short
A Cross-sectional Study of Associations between Adverse Drinking Behaviors and Version).

Abstract
Alcohol drinking is important in the tradition and economy of China. As China holds over 22% of the world's population, the adverse drinking behaviors and health risk behavior in China becomes an important issue in public health. Total 6226 self-administrated questionnaires were collected from newly admitted first year students in two universities in China. My study in 2008 finds family drinking is a predicting factor of binge drinking among male in Shantou; suicidal thought was positively associated with alcohol abuse among Shantou male; internet overuse was positively associated with both alcohol abuse and alcohol dependence among Shantou male; social problem was positively associated with alcohol abuse among Shantou male and female; social problem was positively associated with alcohol dependence among Shantou male; real Body Mass Index (BMI) was positively associated with alcohol abuse among Hong Kong female; and feel depress was positively associated with alcohol abuse among Hong Kong male and female.

VivienLauYW - Stem Cell Therapy

Posted: 11 Dec 2014 05:25 AM PST

VivienLauYW – The Principle, Application and Further Development of Stem Cell Therapy. Laboratory Methods & Instrumentation MMedSc HKU

VivienLauYW – The Principle, Application and Further Development of Stem Cell Therapy. Laboratory Methods & Instrumentation Master of Medical Sciences University of Hong Kong.

VivienLauYW JJY Sung and SM Griffiths - The feasibility of return of self-admitted te

Posted: 11 Dec 2014 05:23 AM PST

The feasibility of return of self-admitted test samples within an agreed duration
V. Y. W Lau1, M. C. S. Wong1,2, J. Y. L. Ching2, S. S. M. Ng2, A. K. C. Luk2, HarryWang1, J. C. Y. Wu2, F. K. L. Chan2, J. J. Y. Sung2 & S. M. Griffiths1
1 School of Public Health and Primary Care, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong SAR, China, 2 Institute of Digestive Disease, Faculty of Medicine, Chinese University of Hong Kong SAR, China

Abstract
Objective: This project is conducted to illustrate the level of consistency of participants who agree to perform a task and the actual completion of the agreed task. In the years 2010 to 2012, a project of colorectal cancer (CRC) recruited 1665 subjects to participate in a project that required them to return two test samples to a center. Faecal Immunochemical Test (FIT) was used in sample collection. Subjects are instructed to return two test samples within 2 weeks, and all 1665 subjects agreed to hand in the two test samples within 2 weeks. However, different levels of actual completion are recorded at the end of the project.

Method: Duration of return of two test samples is recorded by day and month. The trend of the return of test sample number one and number two are recorded separately. Records are counted and presented in percentage. Three trials have been done to improve validity in three subsequence years.

Result: Majority of participants return test sample between day four and day seven in first year and between day eight and day fourteen in next subsequence years. The trend of the return of self-admit test samples increases from the first quarter of a given deadline, peak at the middle of the timeline, decreases a little at third quarter until the date of deadline. 100% participants returned first test sample and 97.84% to 99.51% of participants returned second test sample.

Conclusion: This project demonstrated the effectiveness of self-admitting test samples in an agreed deadline. The compliance of instruction of returning multiple test samples within an agreed time is found feasible and effective in a three-year duration. Such instruction is applicable to projects that require participants to return more than one test samples or questionnaire within an agreed time. Allocation of manpower in collection of test samples for one-year program or first year should load in the mid-point of the timeline. Allocation of manpower should heavily loaded in collection of test samples from mid-point of the timeline to one month beyond the agreed deadline, and phone call reminder should be started one month after the agreed deadline. Allocation of resources is important in increases the cost-effectiveness of research studies.

VivienLauYW and MartinCSWong - The Knowledge of Colorectal Cancer: What hinder colono

Posted: 11 Dec 2014 05:20 AM PST

The Knowledge of Colorectal Cancer: What hinder colonoscopy compliance?
Vivien Y. W. Laua, Martin C. S. Wonga.
a School of Public Health and Primary Care, Faculty of Medicine, Chinese University of Hong Kong, Hong Kong SAR, China

ABSTRACT
Aim: Colonoscopy is a common detection method of bowel cancer, polyps and lesion in digestive tract. Apart from the high sensitivity and specificity on colorectal cancer detection of colonoscopy, there are known risks and long bowel-cleansing preparations about the procedure of colonoscopy. To reduce the waste of resources due to absence of colonoscopy appointment and to encourage public engagement in colonoscopy screening, it is important to collect factors that affect the compliance of colonoscopy. This study investigated the factors that affect the compliance of colonoscopy.

Method: Factors that affect the compliance of colonoscopy were collected in a method of focus groups. A total of 8 focus groups were conducted in 3 months with 48 participants of male and female of age 40-69 with high risk of colorectal cancer who have their colonoscopy appointment arranged in a near future. At the end of each focus group, factors that strongly affecting the compliance of colonoscopy were collected.

Result: Although participants expressed different level of willingness to attend the colonoscopy, all participants completed colonoscopy without delay or absence. P value <0.05 and p value <0.01 were considered statistically significant and all tests were two-tailed. Ninety-five percent confidence intervals were calculated using methods for proportions. The results of 48 participants in 8 focus groups were collected and summarized in this study.

Conclusion: Worry about post-infection and perceiving not necessary for colonoscopy were shown to be the most common factors hindering the decision of completing colonoscopy. Afraid of commitment and afraid to change appointment were shown not to be the major concerns on completing colonoscopy. Most participant stated participation in preparation workshop effectively boosts their confidence to complete colonoscopy. This study shows giving enough information to participants to make decision on colonoscopy is as important as giving guidance to participants on colonoscopy preparation procedure. Allow participants physically and psychologically well prepared for colonoscopy reduces waste of resources due to absence.

VivienLauYW - New Development in molecular epidemiology of infectious diseases

Posted: 11 Dec 2014 05:18 AM PST

New Development in molecular epidemiology of infectious diseases
VivienLauYW

What is Molecular Epidemiology?
Molecular epidemiology contributes to the understanding of the pathogenesis of disease by identifying specific pathways, molecules and genes that influence the risk of developing disease, determining health and disease in families and in populations and the interplay of such molecular factors with environmental factors.

What are Infectious Diseases?
Infectious diseases are caused by pathogenic microorganisms, such as bacteria, viruses, parasites or fungi. The diseases can be spread, directly or indirectly, from one person to another. Infectious diseases costing millions lives globally become an important issue in public health. Infectious diseases such as yellow fever, measles, and tuberculosis are still affecting several parts of the world nowadays. Zoonotic diseases are commonly known in causing disease when transmitted to humans, causing worries since the creation of the world. Commonly known zoonotic diseases are Ebola haemorrhagic fever, Hendra virus and Avian influenza. Ebola haemorrhagic fever causing febrile haemorrhagic illness in human from chimpanzees and porcupines, hendra virus causing fatal respiratory and neurological diseases in human from horses; and Avian influenza causing rapid respiratory deterioration in human from ducks and geese (WHO). However, thanks to the advancement of new technology, the understanding at the molecular level and transmission pattern of infectious disease are increased. Early detection screening is available and targeting-medications and vaccination are investigated; prevention rate and recovery rate are improved. Death rate and treatment fee are reduced. The improvement is profound and wildly reported. Such triumph is encouraging and the awareness of the impact of molecular epidemiology is raised. Successful lowering the prevalence of Plague and Smallpox taught a lesson of global collaboration in molecular epidemiology is essential in eradicating diseases. To further eradicating infectious diseases, new development in molecular epidemiology is in particular focus.

VivienLauYW - List of Nutrient Components could be tested in biologic-clock study

Posted: 11 Dec 2014 05:16 AM PST

List of Nutrient Components could be tested in biologic-clock study
VivienLauYW

In one recent study done in Japanese population, eating behaviors, such as late meal time, eating for a longer time (p<0.005), frequent skip meals (p<0.01) and TV watching at meal (p<0.03), are associated with late sleeping time; however, eating speed was not significantly associated with sleep time (p=0.34) (Sato-Mito et al, 2011). Folate (Vitamin B12,Cobalamin) is abundant in green vegetables (Lee et al, 1998). Vitamin B12, Vitamin B6 and folate, along with zinc and magnesium, facilitate synaptic functioning (Zimmermann, 2001).

VivienLauYW - Guide and Practice

Posted: 11 Dec 2014 05:01 AM PST

Stanford Medical Education
Guide and Practice
Discussion posted about a month ago by VivienLauYW

I agree students need guidance, "Sometimes we need other people who can guide us toward correcting our mistakes and misconceptions." Students then practice and learn from error, "We often are resistant to information that contradicts what we think or know until we disprove it ourselves."